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human plc beta 3 plcβ3  (R&D Systems)


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    R&D Systems human plc beta 3 plcβ3
    Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. <t>PLCβ3,</t> phospholipase C <t>beta</t> 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.
    Human Plc Beta 3 Plcβ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human plc beta 3 plcβ3/product/R&D Systems
    Average 92 stars, based on 2 article reviews
    human plc beta 3 plcβ3 - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry"

    Article Title: Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry

    Journal: Biomedicines

    doi: 10.3390/biomedicines10071574

    Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. PLCβ3, phospholipase C beta 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.
    Figure Legend Snippet: Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. PLCβ3, phospholipase C beta 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.

    Techniques Used: Control, Western Blot, Expressing



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    human plc beta 3 plcβ3  (R&D Systems)
    92
    R&D Systems human plc beta 3 plcβ3
    Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. <t>PLCβ3,</t> phospholipase C <t>beta</t> 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.
    Human Plc Beta 3 Plcβ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human plc beta 3 plcβ3/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    human plc beta 3 plcβ3 - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. PLCβ3, phospholipase C beta 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.

    Journal: Biomedicines

    Article Title: Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry

    doi: 10.3390/biomedicines10071574

    Figure Lengend Snippet: Effects of Klotho (100 pM) and selective TRPC6 inhibitor (BI-749327, 1 μM) on ionic currents of transient receptor potential canonical channel (I TRPC ) in human atrial fibroblasts. ( A ) The current tracings and I–V relationship of I TRP showed that BI-749327-treated fibroblasts (1 μM, 48 h, n = 13–15 from 4 independent experiments), Klotho-treated fibroblasts (100 pM, 48 h, n = 15 from 3 independent experiments), and combined Klotho and BI-749327 ( n = 15 from 3 independent experiments) had similarly lower peak inward currents and similarly lower peak outward currents compared with the control ( n = 13 from 4 independent experiments). ( B ) Western blots showed a similar expression of TRPC6 and total phospholipase C (PLC) between fibroblasts treated with Klotho (100 pM, 48 h) and control fibroblasts. However, the phosphorylated PLC (p-PLC) expression was lower in the fibroblasts treated with Klotho (100 pM, 48 h) ( n = 3 from 3 independent experiments). ( C , D ) Example photographs and average data ( n = 9 from 3 independent experiments) showed that human atrial fibroblasts treated with BI-749327 (1 μM, 48 h), Klotho (100 pM, 48 h), and combined Klotho and BI-749327 exhibited similarly less migratory capability than the control. Arrows indicate migrating fibroblasts in the wound space. GAPDH was used as a loading control. PLCβ3, phospholipase C beta 3; pPLCβ3, phosphorylated phospholipase C beta 3; TRPC6, transient receptor potential canonical channel 6. * p < 0.05, *** p < 0.005.

    Article Snippet: We probed all blots with primary antibodies against procollagen type IA1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), procollagen type III (Abcam, Cambridge, UK), α-smooth muscle actin (α-SMA) (Abcam, Cambridge, UK), TRPC6 (Alomone Labs, Jerusalem, Israel), human PLC beta 3 (PLCβ3) (R&D Systems, Minneapolis, MN, USA), phospho-PLC beta 3 (Ser537) (pPLCβ3 Ser 537) (Cell Signaling Technology, Beverly, MA, USA); secondary antibodies were all conjugated with horseradish peroxidase.

    Techniques: Control, Western Blot, Expressing